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rabbit anti rat secondary antibody  (Vector Laboratories)


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    Structured Review

    Vector Laboratories rabbit anti rat secondary antibody
    Rabbit Anti Rat Secondary Antibody, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1290 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/biotinylated+secondary+rabbit+anti+rat+antibody/Biotinylated+Rabbit+Anti-Rat+IgG+Antibody%2C+mouse+adsorbed/pm42012958-282-31-38
    Average 96 stars, based on 1290 article reviews
    rabbit anti rat secondary antibody - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Polymer:

    Article Title: Globule Leukocytes and other Mast Cells in the Mouse Intestine
    Article Snippet: For detection of ieMMCs, sections underwent antigen retrieval at 100°C for 20 min in Epitope Retrieval solution 2 (ER2) on a Bond Max immunostainer (Leica Biosystems, Bannockburn, IL). .. A rat monoclonal primary antibody to mast cell protease 1 (MCPT1;Cat# 14-5503-82; eBiosciences, San Diego, CA) diluted 1:30 was applied for 15 minutes followed by biotinylated secondary rabbit anti-rat antibody diluted 1:400 (BA-4001; Vector Laboratories, Burlingame, CA) for 10 minutes and then ready-to-use Bond polymer refine detection kit with 3,3’-diaminobenzidine (DAB) as chromogenic substrate and a hematoxylin counterstain (DS9800; Leica Biosystems). .. For detection of lpMMCs and tuft cells, tissue sections underwent antigen retrieval in a prediluted Cell Conditioning Solution ( CC1 ) (Ventana Medical Systems, Indianapolis, IN) for 32 min. For lpMMCs, a goat polyclonal antibody to mast cell protease 4 (MCPT4; LS-B5958; LifeSpan Biosciences, Seattle, WA) was diluted 1:500 and applied for 30 min at RT followed by biotinylated secondary rabbit anti-goat antibody 1:400 (BA-5000; Vector Laboratories) for 10 minutes.

    Article Title: Ixodes ricinus bites promote allergic skin inflammation and intestinal tuft and mast cell expansion in mice
    Article Snippet: Gut sections (5μm) underwent antigen retrieval at 100°C for 20 min in Epitope Retrieval solution 2 (ER2) on a Bond Max immunostainer (Leica Biosystems, Bannockburn, IL). .. A rat monoclonal primary antibody to mast cell protease 1 (MCPT1;Cat# 14-5503-82; eBiosciences, San Diego, CA) diluted 1:30 was applied for 15 minutes followed by biotinylated secondary rabbit anti-rat antibody diluted 1:400 (BA-4001; Vector Laboratories, Burlingame, CA) for 10 minutes and then ready-to-use Bond polymer refine detection kit with 3,3’- diaminobenzidine (DAB) as chromogenic substrate and a hematoxylin counterstain (DS9800; Leica Biosystems). .. For detection of lpMMCs and tuft cells, tissue sections underwent antigen retrieval in a prediluted Cell Conditioning Solution ( CC1 ) (Ventana Medical Systems, Indianapolis, IN) for 32 min. For lpMMCs, a goat polyclonal antibody to mast cell protease 4 (MCPT4; LS-B5958; LifeSpan Biosciences, Seattle, WA) was diluted 1:500 and applied for 30 min at RT followed by biotinylated secondary rabbit anti-goat antibody 1:400 (BA-5000; Vector Laboratories) for 10 minutes.

    Incubation:

    Article Title: HDAC1 and 2 regulate endothelial VCAM-1 expression and atherogenesis by suppressing methylation of the GATA6 promoter
    Article Snippet: After inhibition of endogeneous peroxidase activity with 0.3% H 2 O 2 and blocking of nonspecific binding with normal rabbit serum, tissues were incubated for 90 min with antibody against CD107b (BD Pharmingen, 553322, 1:1000) specifically for macrophages. .. After washing, sections were incubated with biotinylated secondary rabbit anti-rat antibody (Vector Laboratories, BA-4001, 1:200), followed by incubation with avidin-biotin-peroxidase complex (Vector Laboratories, PK-6102). .. The chromogen 3-amino-9-ethyl carbazole (BOSTER Biological Technology, AR1020) was used as the substrate for peroxidase.

    Avidin-Biotin Assay:

    Article Title: HDAC1 and 2 regulate endothelial VCAM-1 expression and atherogenesis by suppressing methylation of the GATA6 promoter
    Article Snippet: After inhibition of endogeneous peroxidase activity with 0.3% H 2 O 2 and blocking of nonspecific binding with normal rabbit serum, tissues were incubated for 90 min with antibody against CD107b (BD Pharmingen, 553322, 1:1000) specifically for macrophages. .. After washing, sections were incubated with biotinylated secondary rabbit anti-rat antibody (Vector Laboratories, BA-4001, 1:200), followed by incubation with avidin-biotin-peroxidase complex (Vector Laboratories, PK-6102). .. The chromogen 3-amino-9-ethyl carbazole (BOSTER Biological Technology, AR1020) was used as the substrate for peroxidase.



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